DIY Biotech Labs are increasingly popular with amateur scientists throughout the world. In this workshop series, participants learn about biohacking - what it is and what it is not, practice different techniques used in molecular biology labs, and learn about DIY Bio citizen scientist projects. $30-$40/workshop depending on supply cost for the laboratory techniques used.
Dates listed below.
Location: STEM Lab (suite 21)
Instructor: Dr. Tonya Shearer
Biohacking 101 and Bioluminescent Bacteria - Thursday, September 13, 10:00am-12:00pm In this workshop, students are introduced to DIY Bio, learn about lab safety and the use of bacteria in molecular biology, and grow bioluminescent bacteria (non-pathogenic, biosafety level 1). $30
Microscopy and Bacterial Characterization - Thursday, October 11, 10:00am-12:00pm Learn microscopy basics including slide preparation, classify bacteria based on colony characteristics, cell shape and Gram stain results, and learn different ways bacteria is grown in a lab. $30
GFP and Bacterial Transformations - Thursday, November 8, 10:00am-12:00pm Green fluorescent protein (GFP) is used as a biomarker in many lab experiments. In this lab, we transform a GFP gene isolated from jellyfish into non-pathogenic bacteria to make the cells glow. $40
DNA Basics and Extraction Techniques - Thursday, December 13, 10:00am-12:00pm DNA is the blueprint for our characteristics and cellular activities. Learn about the structure of DNA, how it is organized into coding and non-coding regions, and practice DNA extraction methods. $30
Polymerase Chain Reaction - Thursday, January 17, 10:00am-12:00pm PCR is a common method to isolate and amplify (make lots of copies of) specific genetic loci from a genome. Participants learn the theory of the polymerase chain reaction, and set up PCRs to amplify a target gene. $40
RFLP and Gel Electrophoresis- Thursday, February 7, 10:00am-12:00pm Participants learn how to break up DNA sequences at restriction sites, and use gel electrophoresis to separate fragments based on size. We discuss when this method is used in science and forensics. $40
DNA Sequencing and Analysis - Thursday, March 14, 10:00am-12:00pm Learn the methods used to determine an organisms DNA sequence, translate DNA codes into amino acid sequences, and search genetic databases to compare DNA sequences. $30
DNA Barcoding - Thursday, April 11, 10:00am-12:00pm In many cases, scientists are able to use a short genetic marker to classify an individual to a specific species. Students practice DNA barcoding methods to identify different organisms, and learn about citizen scientist barcoding projects. $30
Genetic Engineering and CRISPR - Thursday, May 9, 10:00am-12:00pm CRISPR is a gene-editing tool that allows scientists to alter a gene's DNA sequence and influence the gene's function. Students try their hand at CRISPR techniques on non-pathogenic bacteria. $40